Paris, France (SportsNetwork.com) - There won't be a matchup of the Williams sisters at the French Open.
Serena and Venus Williams could have met in the third round at Roland Garros, but, surprisingly, neither made it out of round two on Wednesday.
After Venus was eliminated by Slovakia's Anna Schmiedlova in three sets on Court Chatrier to start the second round, the top-seeded Serena was stunned by Spain's Garbine Muguruza.
Schmiedlova, in her second French Open and fifth Grand Slam event, pulled off a 2-6, 6-3, 6-4 victory over the 29th-seeded Venus for her first-ever win against a top-30 opponent.
The shocker then came about an hour later on Court Lenglen, as Muguruza took full advantage of an uncharacteristically sloppy Serena in a 6-2, 6-2 triumph. Muguruza's best result in five previous Grand Slam events was a fourth-round loss to Agnieszka Radwanska at the Australian Open earlier this year.
It wasn't Serena's earliest exit at Roland Garros. She was bounced in the first round two years ago by Frenchwoman Virginie Razzano, then rebounded by completely dominating her opponents last year en route to her second French title -- dropping just one set in seven matches.
Serena's loss continued the run of surprising outcomes over the last three days in the French capital. Men's third seed Stanislas Wawrinka was sent home in the first round on Monday and Li Na, the women's second seed and 2011 champion, was knocked out Tuesday.
Eighth-seeded German Angelique Kerber avoided an upset Wednesday by tackling American Varvara Lepchenko, 6-2, 7-5.
In other second-round action, Sweden's Johanna Larsson rallied for a surprising 5-7, 6-4, 6-2 victory over 12th-seeded Flavia Pennetta of Italy and 18th-seeded Canadian Eugenie Bouchard came back from a set down to post a 2-6, 6-2, 6-1 win over German Julia Goerges.